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non fat milk  (Proteintech)


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    Structured Review

    Proteintech non fat milk
    Non Fat Milk, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vdac1+2/VDAC1%2F2+Antibody/pmc12993230-67-5-20
    Average 95 stars, based on 180 article reviews
    non fat milk - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Effects of local skeletal muscle cooling prior to acute exercise and endurance training on mitochondrial adaptation in mice.
    Article Snippet: The proteins were then transferred to a polyvinylidene difluoride membrane and blocked with Bullet Blocking One (Nacalai Tesque, Kyoto, Japan). .. The membranes were incubated overnight at 4◦C with primary antibodies diluted 1:1000 in Can Get signal reagent 1 (NKB-201, Toyobo) or TBS-T containing 5% BSA (OXPHOS [#ab110413, abcam], VDAC1/2 [#10866–1-AP, proteintech], COXIV[#ab16056, abcam], SOD1 [#10269–1-AP, proteintech], SOD2 [#ab68155, abcam], Drp1 [#8570, Cell Signaling Technology (CST)], Mfn1 [#13798–1-AP, proteintech]). .. After a 1-h incubation with secondary antibody diluted 1:3000 in TBS-T (anti-rabbit IgG [#7074, CST], anti-mouse IgG [#7076, CST]).

    Article Title: Aerobic exercise improve physical endurance and muscle function by ameliorating oxidative stress and modulating mitochondrial dynamics in aged skeletal muscle.
    Article Snippet: 1 College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China 2 College of Foreign Languages, Zhejiang Normal University, Jinhua 321004, China Abstract Mitochondrial dysfunction is associated with the age-related decline in skeletal muscle mass and strength.. Aerobic exercise upregulate Sestrins protein expression in skeletal muscle, which plays a key role in maintaining mitochondrial homeostasis.. This study aimed to elucidate the role of aerobic exercise in regulating mitochondrial dynamics and oxidative stress in aging skeletal muscle.

    Article Title: Effects of local skeletal muscle cooling prior to acute exercise and endurance training on mitochondrial adaptation in mice
    Article Snippet: The proteins were then transferred to a polyvinylidene difluoride membrane and blocked with Bullet Blocking One (Nacalai Tesque, Kyoto, Japan). .. The membranes were incubated overnight at 4°C with primary antibodies diluted 1:1000 in Can Get signal reagent 1 (NKB-201, Toyobo) or TBS-T containing 5% BSA (OXPHOS [#ab110413, abcam], VDAC1/2 [#10866–1-AP, proteintech], COXIV[#ab16056, abcam], SOD1 [#10269–1-AP, proteintech], SOD2 [#ab68155, abcam], Drp1 [#8570, Cell Signaling Technology (CST)], Mfn1 [#13798–1-AP, proteintech]). .. After a 1-h incubation with secondary antibody diluted 1:3000 in TBS-T (anti-rabbit IgG [#7074, CST], anti-mouse IgG [#7076, CST]).

    Saline:

    Article Title: Aerobic exercise improve physical endurance and muscle function by ameliorating oxidative stress and modulating mitochondrial dynamics in aged skeletal muscle.
    Article Snippet: 1 College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China 2 College of Foreign Languages, Zhejiang Normal University, Jinhua 321004, China Abstract Mitochondrial dysfunction is associated with the age-related decline in skeletal muscle mass and strength.. Aerobic exercise upregulate Sestrins protein expression in skeletal muscle, which plays a key role in maintaining mitochondrial homeostasis.. This study aimed to elucidate the role of aerobic exercise in regulating mitochondrial dynamics and oxidative stress in aging skeletal muscle.

    Pyrolysis Gas Chromatography:

    Article Title: Aerobic exercise improve physical endurance and muscle function by ameliorating oxidative stress and modulating mitochondrial dynamics in aged skeletal muscle.
    Article Snippet: 1 College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China 2 College of Foreign Languages, Zhejiang Normal University, Jinhua 321004, China Abstract Mitochondrial dysfunction is associated with the age-related decline in skeletal muscle mass and strength.. Aerobic exercise upregulate Sestrins protein expression in skeletal muscle, which plays a key role in maintaining mitochondrial homeostasis.. This study aimed to elucidate the role of aerobic exercise in regulating mitochondrial dynamics and oxidative stress in aging skeletal muscle.

    other:

    Article Title: VAMP7-dependent late endosomal secretion of ER and mitochondrial proteins impacts the tumor microenvironment and macrophage engagement.
    Article Snippet: Plasmids used in the study were as follows: pEGFP-C3 (Clonetech Technologies), GFP-VAMP7, RFPLongin (described in 80), pcDNA3.1-Myc-His RTN3A1 was a gift from Jimtong Horng (Addgene plasmid # 31087; http://n2t.net/addgene:31087 ; RRID:Addgene_31087), GFP-CD63 was a gift from John Paul Luzio (Cambridge), Flag-RTN3L was a gift from Ivan Dikic 44, ptfLC3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 21074 ; http://n2t.net/addgene:21074 ; RRID:Addgene_21074).

    Article Title: Polydopamine as a biocompatible and precise mitochondrial targeted therapeutic platform for reversing myocardial ischemia-reperfusion injury
    Article Snippet: MitoSOX red indicator (40778ES50), mitoTracker Deep Red FM (40743ES50), TUNEL apoptosis detection kit (FITC) (40306ES50), annexin V-FITC/PI apoptosis detection kit (40302ES50), and DNA extraction kit (18700ES50) were purchased from Yeasen.

    Western Blot:

    Article Title: VAMP7-dependent late endosomal secretion of ER and mitochondrial proteins impacts the tumor microenvironment and macrophage engagement
    Article Snippet: Normal Rat Kidney (NRK) and Rat differentiated malignant glioma (RG2) cell lines purchased from ATCC were grown at 37 °C and 5% CO 2 in high-glucose DMEM (ThermoFisher, 41966029) supplemented with 10% Fetal Bovine Serum (Biosera, FB-1051H), 2mM L-Glutamine (ThermoFisher, 25030024) and Penicillin-Streptomycin (ThermoFisher, 15070063). .. Primary and secondary antibodies used in the study were as follows: Anti RTN3 (Abcam, ab187764, Western blotting [WB] 1/1000, Immunoflouorescence [IF] 1/250), Anti CD63 (BD Pharmingen, 551458, WB 1/1000, IF 1/250), Anti VDAC1/2 (Proteintech, 55259-1-AP, Abcam, ab14734, WB 1/2000), Anti Myc (Cell Signaling, 2272S, WB 1/1000) Anti Tubulin (Proteintech, 10094-1-AP, WB 1/5000), Anti GFP (Proteintech, 50430-2-AP, WB 1/4000), Anti SNAP47 (SYSY, 111403, WB 1/1000), Anti Syntaxin5 (Gift from Richard Scheller, WB 1/1000), Anti Syntaxin17 (Sigma-Aldrich, HPA001204, WB 1/1000), Anti GRP78/Bip (Proteintech, 11587-1-AP, WB 1/2000), Anti Phospho-eIF2α (Ser51) (Cell Signaling, D9G8, WB 1/1000), Total eIF2α (Cell Signaling, D7D3, WB 1/1000), GAPDH (Sigma-Aldrich, G9545, WB 1/10000), Anti p-S616 DRP1 (Cell Signaling, 4494S, WB 1/1000), Anti p-S637 DRP1 (Cell Signaling, 6319, WB 1/1000), Anti DRP1 (Cell Signaling, 8570S, WB 1/1000), Anti MFF (Cell Signaling, 84580 WB 1/1000), Anti OPA1 (BD Biosciences, 612606, WB 1/1000), Anti MFN2 (Abcam, ab124773, WB 1/1000), Anti Beta-Actin (Sigma-Aldrich, A5441, WB 1/20000), Anti pS2448-mTOR (Cell Signaling, 5536 P, WB 1/1000), Anti mTOR (Cell Signaling, 2983 P, WB 1/1000), Anti LC3B (Abcam, ab48394, WB 1/1000), Anti p62/SQSTM1 (Abcam, ab56416, WB 1/5000), Anti pS757-ULK1 (Cell Signaling, 14202 T, WB 1/1000), Anti ULK1 (Cell Signaling, 8054, WB 1/1000), Anti pT389-P70S6K (Cell Signaling, 9208S, WB 1/1000), P70S6K (Cell Signaling, 9234, WB 1/1000), Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling, 7074, WB 1/10000), Anti-Mouse IgG, HRP- linked Antibody (Cell Signaling, 7076, WB 1/10000), Alexa Fluor 568 Goat Anti-Mouse (ThermoScientific, A11031, IF 1/250), Alexa Fluor 568 Donkey Anti-Mouse (ThermoScientific, A10037, IF 1/250), Alexa Fluor 488 Donkey Anti-Rabbit (ThermoScientific, A21206, IF 1/250), Vimentin (Abcam ab92547, EPR3776, IHC 1/250), Iba1 (WAKO, 019-19741, IHC 1/800). .. Starvation was induced by replacing cell medium with EBSS (ThermoFisher, 24010043) supplemented with 0.2% BSA for 2 hours at 37 °C and 5% CO 2 , followed, in the refeeding condition, by refeeding the cells with full cell medium for 30 min.

    Immunohistochemistry:

    Article Title: VAMP7-dependent late endosomal secretion of ER and mitochondrial proteins impacts the tumor microenvironment and macrophage engagement
    Article Snippet: Normal Rat Kidney (NRK) and Rat differentiated malignant glioma (RG2) cell lines purchased from ATCC were grown at 37 °C and 5% CO 2 in high-glucose DMEM (ThermoFisher, 41966029) supplemented with 10% Fetal Bovine Serum (Biosera, FB-1051H), 2mM L-Glutamine (ThermoFisher, 25030024) and Penicillin-Streptomycin (ThermoFisher, 15070063). .. Primary and secondary antibodies used in the study were as follows: Anti RTN3 (Abcam, ab187764, Western blotting [WB] 1/1000, Immunoflouorescence [IF] 1/250), Anti CD63 (BD Pharmingen, 551458, WB 1/1000, IF 1/250), Anti VDAC1/2 (Proteintech, 55259-1-AP, Abcam, ab14734, WB 1/2000), Anti Myc (Cell Signaling, 2272S, WB 1/1000) Anti Tubulin (Proteintech, 10094-1-AP, WB 1/5000), Anti GFP (Proteintech, 50430-2-AP, WB 1/4000), Anti SNAP47 (SYSY, 111403, WB 1/1000), Anti Syntaxin5 (Gift from Richard Scheller, WB 1/1000), Anti Syntaxin17 (Sigma-Aldrich, HPA001204, WB 1/1000), Anti GRP78/Bip (Proteintech, 11587-1-AP, WB 1/2000), Anti Phospho-eIF2α (Ser51) (Cell Signaling, D9G8, WB 1/1000), Total eIF2α (Cell Signaling, D7D3, WB 1/1000), GAPDH (Sigma-Aldrich, G9545, WB 1/10000), Anti p-S616 DRP1 (Cell Signaling, 4494S, WB 1/1000), Anti p-S637 DRP1 (Cell Signaling, 6319, WB 1/1000), Anti DRP1 (Cell Signaling, 8570S, WB 1/1000), Anti MFF (Cell Signaling, 84580 WB 1/1000), Anti OPA1 (BD Biosciences, 612606, WB 1/1000), Anti MFN2 (Abcam, ab124773, WB 1/1000), Anti Beta-Actin (Sigma-Aldrich, A5441, WB 1/20000), Anti pS2448-mTOR (Cell Signaling, 5536 P, WB 1/1000), Anti mTOR (Cell Signaling, 2983 P, WB 1/1000), Anti LC3B (Abcam, ab48394, WB 1/1000), Anti p62/SQSTM1 (Abcam, ab56416, WB 1/5000), Anti pS757-ULK1 (Cell Signaling, 14202 T, WB 1/1000), Anti ULK1 (Cell Signaling, 8054, WB 1/1000), Anti pT389-P70S6K (Cell Signaling, 9208S, WB 1/1000), P70S6K (Cell Signaling, 9234, WB 1/1000), Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling, 7074, WB 1/10000), Anti-Mouse IgG, HRP- linked Antibody (Cell Signaling, 7076, WB 1/10000), Alexa Fluor 568 Goat Anti-Mouse (ThermoScientific, A11031, IF 1/250), Alexa Fluor 568 Donkey Anti-Mouse (ThermoScientific, A10037, IF 1/250), Alexa Fluor 488 Donkey Anti-Rabbit (ThermoScientific, A21206, IF 1/250), Vimentin (Abcam ab92547, EPR3776, IHC 1/250), Iba1 (WAKO, 019-19741, IHC 1/800). .. Starvation was induced by replacing cell medium with EBSS (ThermoFisher, 24010043) supplemented with 0.2% BSA for 2 hours at 37 °C and 5% CO 2 , followed, in the refeeding condition, by refeeding the cells with full cell medium for 30 min.



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